Affinity Chromatography

Affinity chromatography - Affinity chromatography is a biochemical separation method that combines size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules. This method is usually used to:

Chiral column chromatography - Chiral column chromatography is a variant of column chromatography, where the stationary phase is chiral instead of achiral. The enantiomers of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times.

MRNA display - mRNA Display is a technique used to perform in vitro protein evolution. A synthetic mRNA with puromycin at its 3' end forms an mRNA-peptide fusion that is purified by affinity chromatography.

Countercurrent chromatography - Countercurrent Chromatography(CCC) or Partition Chromatography is a category of liquid-liquid chromatography techniques. Chromatography in general is used to separate components of a mixture based on their differing affinities for mobile and stationary phases of a 'column'.

affinitychromatography

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